English translation for "chloroplast genome"
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- 叶绿体基因组
Related Translations:
- Example Sentences:
| 1. | These include the ability to target the gene of interest to site - specific areas of the chloroplast genome , high - level expression of foreign genes and increased environment security
由于植物叶绿体表达系统能够高效表达目的基因、环境安全性高，已成为目前植物生物反应器研究的热点之一。 | | 2. | Compared with nucleic genome , the chloroplast genome is very small and easily to be manipulated genetically , and the foreign gene is site - direct integrated into the chloroplast genome through homologous recombination
与核转化相比，叶绿体基因组小，遗传操作简单，外源基因是通过同源重组机制定点整合进叶绿体基因组。 | | 3. | In the second part of the thesis , we described that a tobacco chloroplast expression vector , ptrvp1 , containing the foot and mouth disease virus ( fmdv ) vp1 gene and the selective marker aada gene was constructed and transfered to the tobacci chloroplast genome by the biolistic method
论文第二部分主要叙述了烟草叶绿体表达载体ptrvp1的构建，并通过基因枪方法转化烟草叶绿体基因组，获得了3株具有壮观霉素抗性的转化再生植株。 | | 4. | To investigate whether it is suitable for biodegradable plastic production , we constructed the chloroplast integration and expression vector ptrv - phb , and integrated all the genes necessary for phb synthesis into tobacco chloroplast genome through gene - gun transformation . the chloroplast transformants showed phenotypically normal growth and were fertile
本研究在国内率先探讨将叶绿体转化技术引入植物生产生物可降解塑料这一领域的可行性(国外仅有日本一例) ，构建了叶绿体转化及表达载体ptrv - phb ，通过基因枪法将phb合成相关基因导入烟草叶绿体基因组。 | | 5. | To determine that the integration of foreign genes had occurred hi the chloroplast genome at the directed site by homologous recombiantion and to evaluate the degree of homogeneity of the c . reinhardtii transformants , pcr and pcr - southern blot analysis were performed using the primers of c . reinhardtii chloroplast homologous fragment
并根据衣藻叶绿体同源片段chll基因，设计一对引物对其叶绿体dna进行的pcr和southernblot分析。结果表明外源基因已经定点整合入衣藻叶绿体基因组的chll基因中，并且经过三轮抗生素筛选所得的衣藻叶绿体转化子中，转基因型叶绿体基因组拷贝占多数。 | | 6. | To determine the site - specific integration of the foreign gene into the chloroplast genome and the level of homogeneity , the three transformation lines of the second selective generation were analysized through pcr ( pcr - southern blot ) analysis using the primers of fmdv vp1 gene and homologous fragment of tobacco chloroplast , respectively
经过两轮抗生素筛选后，采用fmdvvp1基因的一对引物和烟草叶绿体同源片段的一对引物分别对抗性植株的叶绿体dna进行的pcr和pcr - southernblot分析。 | | 7. | In order to form a chloroplast transformation system of d . salina , we have conducted some studies including its sensitivity to antibiotics , the activity of promoter , cloning of the chloroplast genes and construction of transformation vectors , so far a pilot transformation system of the d . salina chloroplast has been completed . methods : the sensitivity of d . salina to seven antibiotics or herbicide used commonly in gene engineering was studied and the biological activity of atpa promoter from c . reinhardtii chloroplast was tested by using enhanced green fluorescent protein ( egfp ) as a reporter . primers were designed in the conservative encoding regions according to the chloroplast genomes from four algae which have close genetic relationship with d . salina , and the sequences of 16s rrna , chll and chln of d . salina chloroplast were cloned and sequenced , respectively
方法:根据杜氏盐藻的近缘藻类的叶绿体基因组序列资料，在基因编码区的高度保守区域设计引物，克隆了杜氏盐藻叶绿体165识na基因、咖l基因和ch n基因，并分别以165识na基因和chln基因序列为同源片段，以cat和bar基因为筛选标记基因构建了三套杜氏盐藻叶绿体转化载体: 2郑州大学2003年博士学位论文杜氏盐藻( d ~ iiellasalina )叶绿体转化研究pds165一eaf 、 ptn1269一bar和psp72一5一bar一3 ，用基因枪法转化杜氏盐藻，初步建立起杜氏盐藻叶绿体转化体系。 |
- Similar Words：
- "chloroplast dna" English translation, "chloroplast envelope" English translation, "chloroplast fragment" English translation, "chloroplast gene" English translation, "chloroplast genetics" English translation, "chloroplast grana" English translation, "chloroplast membrane system" English translation, "chloroplast messenger rna" English translation, "chloroplast molecular genetics" English translation, "chloroplast mrna" English translation
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